The Definitive Guide to ceesdekker
The Definitive Guide to ceesdekker
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Employing single-molecule optical and AFM imaging and force-spectroscopy procedures, we search for to unravel the mechanistic facets of the composition and dynamics of chromosomes. Our investigation interests include:
It absolutely was only while in the nineteen nineties however, when nanotechnology had State-of-the-art to a stage where solitary molecules could be manipulated, that the primary experimental equipment began to seem.
Multicolor imaging of your bacterial nucleoid and mobile division proteins with blue, orange, and around-infrared fluorescent proteins
Given that 2000, Dekker has shifted the most crucial concentrate of his get the job done towards biophysics in which he scientific tests the properties of single biomolecules and cells using the instruments of nanotechnology. This change of discipline was pushed by his fascination for the exceptional operating of Organic molecular buildings, as well as from the prolonged-time period standpoint that many intriguing discoveries might be predicted During this field.[5] Present traces of investigation in his biophysics team are from the parts of:[six]
2012, discovery that nucleoid occlusion underlies the precision of bacterial cell division; and first at any time research of your dynamics DNA supercoils and the invention of supercoil hopping
Experimental period diagram of negatively supercoiled DNA measured by magnetic tweezers and fluorescence,
‘Initially we checked out Organic units by using a physical watch and acted on the basis on the applications we knew. It took a couple of years in advance of we began Operating additional on the massive biological issues. I then started to phone it nanobiology, a time period that didn't exist at the time.
2011, 1st in vitro measurements of transport across just one ceesdekker biomimetic nuclear pore complicated; improvement of multiplexed magnetic tweezers for kilo-molecule experiments; and resolved the system of homology recognition in DNA homologous recombination
What if, in place of making use of conventional top rated-down silicon electronics, transistors could possibly be built from The underside up utilizing solitary organic molecules as being the switching factors? This concept, which became The idea of these days’s subject of molecular electronics, initial emerged from the 1970s.
There was just one problem although — we did not observe any measurable electrical conduction from the single polymer molecules. We concluded that at the single-molecule stage, conducting polymers ended up basically not this kind of fantastic conductors, which was fairly disappointing. We Hence broadened our look at to test to seek out additional promising molecules.
An built-in microfluidic System for quantifying drug permeation throughout biomimetic vesicle membranes
‘That problems the synthetic cell, as Element of the Dutch BaSyC initiative. Craig Venter seemed major-down in Individuals times and identified about five hundred genes which have been minimally needed for your functioning cell. We wish to look bottom-up: how can we shift from a number of molecules to an entire biological program?
Electrical transport in monolayers of phthalocyanine molecular wires and AFM imaging of an individual wire bridging two electrodes
Good-point out nanopores have proven to become a remarkably flexible probe for solitary-molecule Evaluation of DNA. I will describe a few of our current results – exclusively DNA knots – and also our initiatives to grow the capabilities of sound-state nanopores even additional, within the path of single-protein detection, graphene nanopores, plasmonic nanopores, and DNA origami nanopores.